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2.Identification of a Bacterium Isolated from Galls on Carrot and Weeds

Hideshi Kawarazaki 1, 2・ Masao Goto 1・ Kotaro Kato1 ・ Toshio Kijima1 ・Hiroshi Kawada2 ・ Keisuke Yamamoto2 ・ Yuichi Takikawa2 (1 Institute for Agro-Microbiology, 2 Laboratory of Plant Pathology, Faculty of Agriculture, Shizuoka University)

In 2004, bacterial galls were found on the roots of carrots in Shizuoka Prefecture, Japan. Galls were about 0.1 to 2 cm in diameter, light brown in color and had rough surfaces. In 2005, similar galls were found on the roots of three weeds: henbit (Lamium amplexicaule L.), Persian speedwell (Veronica persica Poir.) and leaf mustard (Brassica juncea L.). A bacterium that forms white, rough colonies was isolated from the carrot and weeds galls. The bacterial isolates had properties identical with Rhizobacter dauci Goto and Kuwata. Phylogenetic analysis based on 16S rDNA sequences showed that the carrot isolate had the highest homology (similarity of 100%) with that of the type strain of R. dauci. Rep-PCR genomic fingerprinting using BOX A1R primer showed that the carrot and weeds isolates were nearly identical. Pathogenicity of the isolates was confirmed by inoculating the roots of carrots and the weeds. After 2-5 weeks, they formed galls on the roots of the original host species and on other plant species tested. The galls were indistinguishable from those formed naturally, and the inoculated bacterium was reisolated. Thus, the causal bacterium of carrot and weeds gall was identified as R. dauci, and the bacterium was found to have a wider host range than previously known. These weed hosts may serve as inoculum sources for carrot bacterial gall disease.

Keywords:Rhizobacter dauci, Bacterial gall, Carrot, Henbit, Persian speedwell, Leaf mustard