RESEARCH REPORTS FROM INSTITUTE FOR AGRO-MICROBIOLOGY, Vol. 12, 2008 p.43-59
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3.Isolation of Endophytic Bacteria Promoting the Resistance to Bacterial Wilt and the Growth of Tomato

Yoshiyuki Nara*1, 3・Kotaro Kato*3・Hideshi Kawarazaki*3・Hiroyasu Tabuchi*3 Masao Goto*3・Tohru Teraoka*2・Tsutomu Arie*2・Toshio Kijima*3 （*1United Graduate School of Agriculture Science, *2 Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology (TUAT), *3Institute for Agro-Microbiology,）

Two hundreds and forty-five plant-associated bacteria isolated from soil by techniques including hypocotyl trapping were subjected to screening based on promotion of resistance against bacterial wilt and growth in tomato. Three isolates, KSR01, KSB07 and KLT08, were selected. When tomato seeds were treated with KSR01, growth was promoted and the disease severity by bacterial wilt was reduced significantly in comparison to the non-treated control. KSR01 was re-isolated from the stem tissues of tomato seed-treated with KSR01 35 days after treatment, suggesting that KSR01 was endophytic in tomato. KSR01 did not produce antibacterial substances against Ralstonia solanacearum, the pathogen of bacterial wilt. This suggested that the mode of action of KSR01 against bacterial wilt was induction of resistance. KSR01 was identified as Herbaspirillum huttiensis by fatty acid analysis, 16S rDNA sequence and physiological and biochemical characteristics.

Keywords:Endophyte, Biological control, Plant-growth promotion, Bacterial wilt, Ralstonia solanacearum